Country: United Kingdom

Location: Cambridge

Organisation: Wellcome Trust - Medical Research Council Cambridge Stem Cell Institute, University of Cambridge

Post-Doctoral Research Associate (Fixed Term)
Salary: £29,301-£38,183
Reference: PS11207
Closing date: 24 February 2017

Fixed-term: The funds for this post are available until 31 January 2020 in the first instance.
Applications are invited for a postdoctoral position to explore experimentally the earliest steps of pre-neoplastic transformation in the epithelial tissues. 

The position, which is supported by a Multidisciplinary Project Award from the CRUK, will be hosted in the Cambridge Stem Cell Institute (CSCI) and is associated with the research groups of Prof. Benjamin Simons (Physics/CSCI) and Dr. Bon-Kyoung Koo (Genetics/CSCI). 

This joint project aims at investigating the impact of tumorigenic mutations in the clone evolution from pre-neoplasia to invasive carcinoma, with an emphasis of the gastrointestinal tract. In order to advance our understanding in this field, we have developed novel variants of the Rosa-Confetti 4-colour reporter allele. These variants harbour different oncogenes, site-specific recombinase or Cas9 endonuclease coupled to a red fluorescent protein of the Rosa-Confetti lineage tracing system. Drawing upon developments in mathematical modelling, advanced imaging and image-analysis, we will define the clonal dynamics of tumorigenic mutation-containing clones as well as the response of surrounding normal clones in different adult tissues under different conditions: homeostasis, regeneration and/or inflammation. At the same time, we will perform Cas9-assisted in vivo genetic screening coupled with statistical analysis and imaging processing to determine genes associated with pre-neoplastic field cancerisation. 

The successful applicant will lead the biological analysis of established lineage tracing models with confocal or multiphoton imaging analysis of near naïve epithelial tissues under the joint supervision of Dr Bon-Kyoung Koo and Prof Benjamin Simons. Quantitative biophysical analysis will be supported by the group of Prof Benjamin Simons. 

Homepage: http://www.stemcells.cam.ac.uk/researchers/principal-investigators/bon-… 
http://www.stemcells.cam.ac.uk/researchers/principal-investigators/pres… 

Requirement: 

  • Final year PhD student or PhD in relevant field.
  • Strong background in molecular biology and mouse genetics. 
  • Knowledge in molecular cloning, lineage tracing, confocal imaging and image processing/analysis. 
  • Experience in epithelial biology with murine genetic models. 
  • Experience in mouse ESC culture, mouse line generation and mouse work. 
  • Experience in tissue clearing, fluorescence imaging, fluorescence activated cell analysis and cell sorting. 
  • A proven capacity to design, execute and correctly interpret your own experiments. 
  • Good verbal and written communication skills in English. 
  • Highly independent, motivated, ambitious, and collaborative person. 

To apply online for this vacancy and to view further information about the role, please visit: http://www.jobs.cam.ac.uk/job/12653. This will take you to the role on the University’s Job Opportunities pages. There you will need to click on the 'Apply online' button and register an account with the University's Web Recruitment System (if you have not already) and log in before completing the online application form.

The closing date for all applications is Friday 24 February 2017.

Please upload your Curriculum Vitae (CV) and a covering letter in the Upload section of the online application to supplement your application. If you upload any additional documents which have not been requested, we will not be able to consider these as part of your application. 

Informal enquiries are also welcome via email: jobs@stemcells.cam.ac.uk.

Interviews will be held in March 2017. If you have not been invited for interview by 31 March 2017, you have not been successful on this occasion.

Please quote reference PS11207 on your application and in any correspondence about this vacancy.

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